Difference between revisions of "Part:BBa K1141001:Design"

 
(Adjusted design notes.)
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1141001 short</partinfo>
 
<partinfo>BBa_K1141001 short</partinfo>
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===Design Notes===
 
===Design Notes===
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None
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The original restriction sites of the pQE30 plasmid weren't removed from the biobrick in this version: these restriciton sites are the Eco RI and Bam HI sites annotated in the sequence.
  
  

Latest revision as of 13:02, 23 September 2013

Plac-RBS-KillerRed (IPTG-inducible, pQE30 plasmid format)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 82
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 82
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 82
    Illegal BamHI site found at 139
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 82
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 82
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 295
    Illegal BsaI.rc site found at 586


Design Notes

The original restriction sites of the pQE30 plasmid weren't removed from the biobrick in this version: these restriciton sites are the Eco RI and Bam HI sites annotated in the sequence.


Source

The source of this part is the pQE30 plasmid from QIAGEN (for PLac-RBS) and the KillerRed sequence from INVITROGEN. KillerRed was originally engineered from the anm2cp anthomedusa chromoprotein by Maria E. Bulina et al.

References