Difference between revisions of "Part:BBa K1073001"
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<partinfo>BBa_K1073001 short</partinfo>
 
<partinfo>BBa_K1073001 short</partinfo>
  
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This construct is derived from BBa_R0071 and BBa_B0032. It combines the inducible promoter of the rhl Quorum sensing system of ''P. aeruginosa'' which is activated by RhlR in combination with N-buturyl homoserine lactone (N-buturyl-HSL) with an RBS. The device can be cloned in front of a desired gene of interest in order to induce its Expression by N-buturyl-HSL.
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This construct is derived from BBa_R0071 and BBa_B0032. It combines the inducible promoter of the rhl Quorum sensing system of ''P. aeruginosa'' which is activated by RhlR in combination with N-buturyl homoserine lactone (N-buturyl-HSL) with an RBS.  
  
 
<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
===Usage and Biology===
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The inducible Promotor from ''Pseudomonas aeruginosa'' is induced by RhlR complexed with the specific autoinducer butanoyl-homoserine lactone produced by RhlI. In ''Pseudomonas aeruginosa'' the induction of the promoter leads to activation of transcription of different virulence-associated traits in the cell [Medina 2003].  
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The inducible Promotor from ''Pseudomonas aeruginosa'' is induced by RhlR complexed with the specific autoinducer N-buturyl-HSL produced by RhlI. In ''Pseudomonas aeruginosa'' the induction of the promoter leads to activation of transcription of different virulence-associated traits in the cell [Medina 2003].
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The device can be cloned in front of a desired gene of interest in order to induce its Expression by N-buturyl-HSL.
 
For further Information see BBa_R0071.
 
For further Information see BBa_R0071.
  

Revision as of 08:02, 23 September 2013

Inducible promoter of the rhl quorum sensing system with RBS

This construct is derived from BBa_R0071 and BBa_B0032. It combines the inducible promoter of the rhl Quorum sensing system of P. aeruginosa which is activated by RhlR in combination with N-buturyl homoserine lactone (N-buturyl-HSL) with an RBS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]