Difference between revisions of "Part:BBa K1080007:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | Incorporated sequence overlap for Gibson assembly and no GC rich region or restriction site in sequence | |
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===Source=== | ===Source=== |
Latest revision as of 01:51, 23 September 2013
POR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 600
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 139
Illegal AgeI site found at 190 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Incorporated sequence overlap for Gibson assembly and no GC rich region or restriction site in sequence
Source
Chlamydomonas reinhardtii