Difference between revisions of "Part:BBa K1080005:Design"

(→‎Design Notes)
(→‎Design Notes)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
 +
Incorporated sequence overlap for Gibson assembly and no GC rich region or restriction site in sequence
  
 
===Source===
 
===Source===

Revision as of 01:50, 23 September 2013


CTH1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 305
    Illegal BglII site found at 413
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 103
    Illegal BsaI.rc site found at 259
    Illegal BsaI.rc site found at 880


Design Notes

Incorporated sequence overlap for Gibson assembly and no GC rich region or restriction site in sequence

Source

Chlamydomonas reinhardtii

References