Difference between revisions of "Part:BBa K1080000:Design"

(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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 +
Incorporated sequence overlap for Gibson assembly and no GC rich region or restriction site in sequence
  
 
===Source===
 
===Source===

Revision as of 01:40, 23 September 2013


ChlI1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 231
    Illegal BglII site found at 887
    Illegal BglII site found at 1082
    Illegal BamHI site found at 279
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Incorporated sequence overlap for Gibson assembly and no GC rich region or restriction site in sequence

Source

Chlamydomonas reinhardtii

References