Difference between revisions of "An Introduction to BioBricks"

Revision as of 21:55, 13 July 2006

BioBricks are a set of interchangable parts, designed with a view to building biological systems in living cells. The picture to the left shows BioBrick part BBa_0034 within a plasmid. The yellow arrow represents the plasmid's antibiotic resistance gene and the green arrow, its origin of replication. For more information see:

Building BioBrick Systems

BioBrick parts can be assembled to form useful devices, through a process often refeed to as 'Standard Assembly' This uses normal cloning techniques based on restriction enzymes, purification, ligation, and transformation. BioBrick parts are composable; allowing endless numbers of biobricks to be pieced together to form higher systems.

The assembly to the left shows 3 BioBricks assembled to create a simple device. You can learn more about this device by following the link to its associated page in the registry. You may like look into:

Standard Assembly - The classical method of BioBrick assembly, done at the lab bench
BioBrick Restriction Enzymes
Rolling Assembly
Automated assembly - currently in late stage development at iGEM labs

How do I make a BioBrick?

Adding the part to the registry

This protocol is quick and easy. A comprehensive guide can be found here

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 BioBricks are a set of interchangable parts, designed with a view to building biological systems in living cells. The picture to the left shows BioBrick part [[Part:BBa_B0034|BBa_0034]] within a [[Help:Plasmids|plasmid]]. The yellow arrow represents the plasmid's antibiotic resistance gene and the green arrow, its origin of replication.  For more information see: