Difference between revisions of "Part:BBa K1163101"
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| − | This promoter allows you to have an iron-sensitive behaviour. In fact, in the presence of iron, the | + | This promoter allows you to have an iron-sensitive behaviour. In fact, in the presence of iron, the AceB promoter region decreases the expression of the downstream gene. |
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Revision as of 19:43, 22 September 2013
pAceB promoter region
Promoter from E. coli that controls the AceB gene, involved in iron uptake and iron sensitive.
FUR (Ferric Uptake Regulation) is a transcriptional repressor of genes involved in iron homeostasis. In presence of iron, the FUR protein changes its conformation and dimerizes. This leads to the binding to the DNA on a FUR binding site, thus inhibiting the mRNA transcription.
Why would you use this part?
This promoter allows you to have an iron-sensitive behaviour. In fact, in the presence of iron, the AceB promoter region decreases the expression of the downstream gene.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 277
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]