Difference between revisions of "An Introduction to BioBricks"

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(→‎At the bench)
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==How do I make a BioBrick?==
 
==How do I make a BioBrick?==
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====At the bench====
 
  
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Pic of biobrick flanked by coding seq
 
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Methods:
 
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*Synthesis
 
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*PCR
 
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More information on putting sequence into standardized Biobrick form can be found [[Help:Standardization|here]]
 
  
 
====Adding the part to the registry====
 
====Adding the part to the registry====
 
This protocol is quick and easy. A comprehensive guide can be found [[Basic Biobrick, example 1|here]]
 
This protocol is quick and easy. A comprehensive guide can be found [[Basic Biobrick, example 1|here]]

Revision as of 19:55, 13 July 2006

Partinplasmid.png

BioBricks are a set of interchangable parts, designed with a view to building biological systems in living cells. The picture to the left shows BioBrick part BBa_0034 within a plasmid. The yellow arrow represents the plasmid's antibiotic resistance gene and the green arrow, its origin of replication. For more information see:


Building BioBrick Systems

MultiPartsinplasmid.png

BioBrick parts can be assembled to form useful devices, through a process often refeed to as 'Standard Assembly' This uses normal cloning techniques based on restriction enzymes, purification, ligation, and transformation. BioBrick parts are composable; allowing endless numbers of biobricks to be pieced together to form higher systems.

The assembly to the left shows 3 BioBricks assembled to create a simple device. You can learn more about this device by following the link to its associated page in the registry. You may like look into:

How do I make a BioBrick?

Adding the part to the registry

This protocol is quick and easy. A comprehensive guide can be found here