Difference between revisions of "Part:BBa K1017401"

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The DNA sequence produces sRNA which can combine with the sequence of its target region on the rRBS-1 designed by us, blocking the gene expressions downstream. It is the temporary design which can be simply amplified.
 
The DNA sequence produces sRNA which can combine with the sequence of its target region on the rRBS-1 designed by us, blocking the gene expressions downstream. It is the temporary design which can be simply amplified.
  
[[File:NCTU_sRNA-synthesis.png]]
+
[[File:NCTU_sRNA-synthesis.png|center|600px]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 19:27, 22 September 2013

sRNA+rRBS-1


The DNA sequence produces sRNA which can combine with the sequence of its target region on the rRBS-1 designed by us, blocking the gene expressions downstream. It is the temporary design which can be simply amplified.

NCTU sRNA-synthesis.png

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 144
    Illegal SpeI site found at 129
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 129
    Illegal NotI site found at 135
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 147
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 144
    Illegal SpeI site found at 129
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 144
    Illegal SpeI site found at 129
  • 1000
    COMPATIBLE WITH RFC[1000]