Difference between revisions of "An Introduction to BioBricks"

(Building BioBrick Systems)
(Building BioBrick Systems)
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The assembly to the left shows 3 BioBricks assembled to create a simple device. You can learn more about this device by following the link to its associated page in the registry. You may like look into:
 
The assembly to the left shows 3 BioBricks assembled to create a simple device. You can learn more about this device by following the link to its associated page in the registry. You may like look into:
*[[Assembly:Robotic assembly|Standard Assembly]] - <i>The classical method of BioBrick assembly, done at the lab bench.
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*[[Assembly:Standard assembly|Standard Assembly]] - <i>The classical method of BioBrick assembly, done at the lab bench</i>
 
*[[Assembly:Restriction enzymes|BioBrick Restriction Enzymes]]
 
*[[Assembly:Restriction enzymes|BioBrick Restriction Enzymes]]
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*[[Assembly:Rolling assembly|Rolling Assembly]]
 
*[[Assembly:Robotic assembly| Automated assembly]] - <i>currently in late stage development at iGEM labs</i>
 
*[[Assembly:Robotic assembly| Automated assembly]] - <i>currently in late stage development at iGEM labs</i>
 
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Revision as of 19:55, 13 July 2006

Partinplasmid.png

BioBricks are a set of interchangable parts, designed with a view to building biological systems in living cells. The picture to the left shows BioBrick part BBa_0034 within a plasmid. The yellow arrow represents the plasmid's antibiotic resistance gene and the green arrow, its origin of replication. For more information see:


Building BioBrick Systems

MultiPartsinplasmid.png

BioBrick parts can be assembled to form useful devices, through a process often refeed to as 'Standard Assembly' This uses normal cloning techniques based on restriction enzymes, purification, ligation, and transformation. BioBrick parts are composable; allowing endless numbers of biobricks to be pieced together to form higher systems.

The assembly to the left shows 3 BioBricks assembled to create a simple device. You can learn more about this device by following the link to its associated page in the registry. You may like look into:

How do I make a BioBrick?

At the bench

Pic of biobrick flanked by coding seq

Methods:

  • Synthesis
  • PCR

More information on putting sequence into standardized Biobrick form can be found here

Adding the part to the registry

This protocol is quick and easy. A comprehensive guide can be found here