Difference between revisions of "An Introduction to BioBricks"
Jamesbrown (Talk | contribs) (→Building BioBrick Systems) |
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The assembly to the left shows 3 BioBricks assembled to create a simple device. You can learn more about this device by following the link to its associated page in the registry. You may like look into: | The assembly to the left shows 3 BioBricks assembled to create a simple device. You can learn more about this device by following the link to its associated page in the registry. You may like look into: | ||
− | *[[Assembly: | + | *[[Assembly:Standard assembly|Standard Assembly]] - <i>The classical method of BioBrick assembly, done at the lab bench</i> |
*[[Assembly:Restriction enzymes|BioBrick Restriction Enzymes]] | *[[Assembly:Restriction enzymes|BioBrick Restriction Enzymes]] | ||
+ | *[[Assembly:Rolling assembly|Rolling Assembly]] | ||
*[[Assembly:Robotic assembly| Automated assembly]] - <i>currently in late stage development at iGEM labs</i> | *[[Assembly:Robotic assembly| Automated assembly]] - <i>currently in late stage development at iGEM labs</i> | ||
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Revision as of 19:55, 13 July 2006
BioBricks are a set of interchangable parts, designed with a view to building biological systems in living cells. The picture to the left shows BioBrick part BBa_0034 within a plasmid. The yellow arrow represents the plasmid's antibiotic resistance gene and the green arrow, its origin of replication. For more information see: |
Building BioBrick Systems
BioBrick parts can be assembled to form useful devices, through a process often refeed to as 'Standard Assembly' This uses normal cloning techniques based on restriction enzymes, purification, ligation, and transformation. BioBrick parts are composable; allowing endless numbers of biobricks to be pieced together to form higher systems. The assembly to the left shows 3 BioBricks assembled to create a simple device. You can learn more about this device by following the link to its associated page in the registry. You may like look into:
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How do I make a BioBrick?
At the bench
Pic of biobrick flanked by coding seq
Methods:
- Synthesis
- PCR
More information on putting sequence into standardized Biobrick form can be found here
Adding the part to the registry
This protocol is quick and easy. A comprehensive guide can be found here