Difference between revisions of "Part:BBa K1157021"

Latest revision as of 16:23, 22 September 2013

pRhl-RhlR-GFP-Pc-RhlR

This is a biosensor with positive-feedback design, for sensing C-4 AHL molecules(a type of quorum sensing(QS) molecule) produced by Psuedomonas aeruginosa. We use a constitutive promoter to express Rhl receptor, and when C-4 AHL binds with Rhl receptor(RhlR), it will bind on its specific promoter sequence pRhl, and regulate the expression of RhlR and GFP. By sensing the GFP signal, we are able to detect QS signals.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1712
Illegal NheI site found at 1735
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 322
Illegal BamHI site found at 2009
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 797
Illegal BsaI site found at 2484
Illegal BsaI.rc site found at 1484

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 __NOTOC__
 <partinfo>BBa_K1157021 short</partinfo>
-This is a biosensor for C-4 AHL molecules(a type of quorum sensing(QS) molecule) produced by Psuedomonas aeruginosa. We use a constitutive promoter to express Rhl receptor, and when C-4 AHL binds with Rhl receptor(RhlR), it will combine with its specific promoter sequence pRhl and regulate the expression of producing more RhlR and GFP. By sensing the GFP signal, we are able to detect QS signals.
+This is a biosensor with positive-feedback design, for sensing C-4 AHL molecules(a type of quorum sensing(QS) molecule) produced by Psuedomonas aeruginosa. We use a constitutive promoter to express Rhl receptor, and when C-4 AHL binds with Rhl receptor(RhlR), it will bind on its specific promoter sequence pRhl, and regulate the expression of RhlR and GFP. By sensing the GFP signal, we are able to detect QS signals.
 <!-- Add more about the biology of this part here