Difference between revisions of "Part:BBa K1157013"

Revision as of 16:01, 22 September 2013

pCI-mCherry-pConst-RhlR-pRhl-RhlR-CI

This biosensor is constructed to sense C-4 AHL molecules through a method different from BBa_K1157012. This time when AHL molecules are sensed, it activates Rhl promoter, which initiates the production of CI. Therefore, CI inhibits pCI and the expression of reporter mCherry is stopped. When compared to control group, the results will be expected to express lower fluorescence level when there is existence of AHL molecules.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 944
Illegal NheI site found at 967
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1241
Illegal BamHI site found at 2197
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1716
Illegal BsaI site found at 2672

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1157013 short</partinfo>
-This biosensor is constructed to sense C-4 AHL molecules through a method different from BBa_K1157012. This time when AHL molecules are sensed, it produces Rhl receptors which initiates production of CI. Therefore, CI inhibits pCI and the expression of reporter mCherry. When compared to control group, the results will be expected to express lower fluorescence level when there is existence of AHL molecules.
+This biosensor is constructed to sense C-4 AHL molecules through a method different from BBa_K1157012. This time when AHL molecules are sensed, it activates Rhl promoter, which initiates the production of CI. Therefore, CI inhibits pCI and the expression of reporter mCherry is stopped. When compared to control group, the results will be expected to express lower fluorescence level when there is existence of AHL molecules.
 <!-- Add more about the biology of this part here