Difference between revisions of "Part:BBa K1017602"

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	<partinfo>BBa_K1017602 short</partinfo>		<partinfo>BBa_K1017602 short</partinfo>
−	This biobrick is comprised of  37 degrees Celsius RBS and mGFP. 37℃ RBS, a RBS which on/off is controlled by temperature. mRFP is a red fluorescent protein. When the temperature is higher than 37℃, 37℃ RBS functions normally and the downstream gene can be expressed. In contrast with high temperature,37℃ RBS forms a hairpin structure that block the ribosome binding when lower 37℃. Thus, the downstream gene, mGFP, is not able to translate.	+	This biobrick is comprised of  37 degrees Celsius RBS and mGFP. 37℃ RBS, a RBS which on/off is controlled by temperature. mRFP is a red fluorescent protein. <br>When the temperature is higher than 37℃, 37℃ RBS functions normally and the downstream gene can be expressed. In contrast with high temperature,37℃ RBS forms a hairpin structure that block the ribosome binding when lower 37℃. Thus, the downstream gene, mGFP, is not able to translate.[[File:NCTU_RNAthermometer.jpg|center|Fig 1. When the temperature increases, the secondary structure near the SD site would change its conformation and allow the ribosome to bind to initiate translation]]
		+	<br>From chart 1., we can clearly found that the time to reach same O.D. value(0.8) various as the temperature changes.
		+	[[File:NCTU_37RBS_result.png|center|600px|Chart 1.37°C RBS efficiency under different temperatures.]]
		+
	<!-- Add more about the biology of this part here		<!-- Add more about the biology of this part here
	===Usage and Biology===		===Usage and Biology===

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Revision as of 09:31, 22 September 2013

37℃ RBS + mGFP

This biobrick is comprised of 37 degrees Celsius RBS and mGFP. 37℃ RBS, a RBS which on/off is controlled by temperature. mRFP is a red fluorescent protein.
When the temperature is higher than 37℃, 37℃ RBS functions normally and the downstream gene can be expressed. In contrast with high temperature,37℃ RBS forms a hairpin structure that block the ribosome binding when lower 37℃. Thus, the downstream gene, mGFP, is not able to translate.

From chart 1., we can clearly found that the time to reach same O.D. value(0.8) various as the temperature changes.

Sequence and Features