Difference between revisions of "Part:BBa K1100015:Design"

(Source)
(Source)
Line 14: Line 14:
  
 
R0010 from Kit,
 
R0010 from Kit,
Csy4 loci via PCR,
+
Insulator: Csy4 loci via PCR,
 
sfGFP from Ding Yu lab at Fudan University
 
sfGFP from Ding Yu lab at Fudan University
  

Revision as of 11:49, 21 September 2013


R0010 with insulator and sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

coming soon


Source

R0010 from Kit, Insulator: Csy4 loci via PCR, sfGFP from Ding Yu lab at Fudan University

References

Qi L, Haurwitz R E, Shao W, et al. RNA processing enables predictable programming of gene expression[J]. Nature biotechnology, 2012, 30(10): 1002-1006. Haurwitz R E, Jinek M, Wiedenheft B, et al. Sequence-and structure-specific RNA processing by a CRISPR endonuclease[J]. Science, 2010, 329(5997): 1355-1358.