Difference between revisions of "Part:BBa K1154005:Design"
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===Design Notes===
 
===Design Notes===
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The 5'-terminal 403 nucleotides are from ''Saccharomyces cerevisiae'' Reference Strain S288C chromosome II, coordinates 477268 to 477670, in the promoter region of the TEF2/YBR118W coding sequence. That sequence is identical to [https://parts.igem.org/Part:BBa_K165037 Part BBa_K165037]. This variant has an additional six nucleotides (tactag) added to the 3' terminus.
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The 5'-terminal 403 nucleotides are from ''Saccharomyces cerevisiae'' Reference Strain S288C chromosome II, coordinates 477268 to 477670, in the promoter region of the TEF2/YBR118W coding sequence. That sequence is identical to [https://parts.igem.org/Part:BBa_K165037 Part BBa_K165037]. This variant has an additional six nucleotides (tactag) added to its 3' terminus.
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===Source===
 
===Source===

Latest revision as of 04:20, 21 September 2013

Saccharomyces cerevisiae constitutive TEF2 promoter variant


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The 5'-terminal 403 nucleotides are from Saccharomyces cerevisiae Reference Strain S288C chromosome II, coordinates 477268 to 477670, in the promoter region of the TEF2/YBR118W coding sequence. That sequence is identical to Part BBa_K165037. This variant has an additional six nucleotides (tactag) added to its 3' terminus.

Source

Sequence is from the genome of Saccharomyces cerevisiae Reference Strain S288C.

References

Please consult the [http://www.yeastgenome.org/ Saccharomyces Genome Database] for copius information and references.