Difference between revisions of "Part:BBa K1017301:Design"
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Levskaya, A. et al (2005). Engineering Escherichia coli to see light. Nature, 438(7067), 442. | Levskaya, A. et al (2005). Engineering Escherichia coli to see light. Nature, 438(7067), 442. | ||
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2012 ETH Zurich iGEM team | 2012 ETH Zurich iGEM team | ||
Latest revision as of 02:39, 21 September 2013
Cph8(cph1-envZ)
Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 364
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Since OmpR, the regulator of PompC, should be phosphorylated to turn on the promoter, we use the photoreceptor cph1(with PCB) to sense the light, then determine whether OmpR is active.
Source
Synthesis
References
Levskaya, A. et al (2005). Engineering Escherichia coli to see light. Nature, 438(7067), 442.
2012 ETH Zurich iGEM team