Difference between revisions of "Part:BBa K1017301:Design"
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===References===
 
===References===
 +
 +
Levskaya, A. et al (2005). Engineering Escherichia coli to see light. Nature, 438(7067), 442.
 +
2012 ETH Zurich iGEM team

Revision as of 02:39, 21 September 2013

Cph8(cph1-envZ)


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Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 364
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since OmpR, the regulator of PompC, should be phosphorylated to turn on the promoter, we use the photoreceptor cph1(with PCB) to sense the light, then determine whether OmpR is active.


Source

Synthesis

References

Levskaya, A. et al (2005). Engineering Escherichia coli to see light. Nature, 438(7067), 442. 2012 ETH Zurich iGEM team