Difference between revisions of "Part:BBa K1132028:Design"
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<partinfo>BBa_K1132028 short</partinfo> | <partinfo>BBa_K1132028 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | + | Bxb1 integrase allows DNA 180° switching between two recognition sites (attB and attP). See BBa_K907000 for further infomation. | |
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===Source=== | ===Source=== | ||
| − | + | BBa_B0034 (RBS),<br> BBa_K907000 (Bxb1 integrase) | |
===References=== | ===References=== | ||
Latest revision as of 12:34, 20 September 2013
Bxb1 integrase coding sequence with RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 210
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 484
Illegal XhoI site found at 571 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1123
Illegal NgoMIV site found at 1210
Illegal AgeI site found at 260 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1318
Design Notes
Bxb1 integrase allows DNA 180° switching between two recognition sites (attB and attP). See BBa_K907000 for further infomation.
Source
BBa_B0034 (RBS),
BBa_K907000 (Bxb1 integrase)