Difference between revisions of "Part:BBa K1132027:Design"
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<partinfo>BBa_K1132027 short</partinfo> | <partinfo>BBa_K1132027 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
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| + | Bxb1 integrase allows DNA 180° switching between two recognition sites (attB and attP). As the protein is very active even at low level of expression, it is associated with a constitutive weak promoter. See BBa_K907000 for further infomation. | ||
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===Source=== | ===Source=== | ||
| − | + | Bxb1 integrase allows DNA 180° switching between two recognition sites (attB and attP). As the protein is very active even at low level of expression, it is associated with a constitutive weak promoter. See BBa_K907000 for further infomation. | |
===References=== | ===References=== | ||
Revision as of 12:30, 20 September 2013
Bxb1 integrase coding sequence with constitutive weak promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 253 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 527
Illegal XhoI site found at 614 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1166
Illegal NgoMIV site found at 1253
Illegal AgeI site found at 303 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1361
Design Notes
Bxb1 integrase allows DNA 180° switching between two recognition sites (attB and attP). As the protein is very active even at low level of expression, it is associated with a constitutive weak promoter. See BBa_K907000 for further infomation.
Source
Bxb1 integrase allows DNA 180° switching between two recognition sites (attB and attP). As the protein is very active even at low level of expression, it is associated with a constitutive weak promoter. See BBa_K907000 for further infomation.