Difference between revisions of "Part:BBa K1132025"

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FimE integrase allows DNA 180° switching between two recognition sites (IRL : BBa_K137010 and IRR : BBa_K137008). As the protein is very active even at low level of expression, it is associated with a constitutive weak promoter.
  
 
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Latest revision as of 12:25, 20 September 2013

FimE integrase coding sequence with constitutive weak promoter and terminator.

FimE integrase allows DNA 180° switching between two recognition sites (IRL : BBa_K137010 and IRR : BBa_K137008). As the protein is very active even at low level of expression, it is associated with a constitutive weak promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]