Difference between revisions of "Part:BBa K1017101"
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Pred, including PompC,RBS(B0032), LacI, ter(J61048), and Plac, can be activated when exposed to red light.  OmpR, the transcription factor of PompC, is active when being phosphorylated in dark and inactive under red light. When there is red light, the non phosphorylated OmpR cannot turn on PompC. Thus, Plac won't be repressed by the negative regulator, LacI. So the whole Pred can express genes downstream Plac. On the other hand, if there is no red light, the phosphorylated OmpR then turn on PompC, causing LacI to be expressed and repress Plac to translate the genes downstream.
 
Pred, including PompC,RBS(B0032), LacI, ter(J61048), and Plac, can be activated when exposed to red light.  OmpR, the transcription factor of PompC, is active when being phosphorylated in dark and inactive under red light. When there is red light, the non phosphorylated OmpR cannot turn on PompC. Thus, Plac won't be repressed by the negative regulator, LacI. So the whole Pred can express genes downstream Plac. On the other hand, if there is no red light, the phosphorylated OmpR then turn on PompC, causing LacI to be expressed and repress Plac to translate the genes downstream.
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[[image:Nctu_formosa_pred.png|300px]]
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https://static.igem.org/mediawiki/2013/c/c7/Nctu_formosa_pred.png
  
 
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Revision as of 10:33, 20 September 2013

Pred ( PompC + B0032 + LacI + J61048 + Plac)

Pred, including PompC,RBS(B0032), LacI, ter(J61048), and Plac, can be activated when exposed to red light. OmpR, the transcription factor of PompC, is active when being phosphorylated in dark and inactive under red light. When there is red light, the non phosphorylated OmpR cannot turn on PompC. Thus, Plac won't be repressed by the negative regulator, LacI. So the whole Pred can express genes downstream Plac. On the other hand, if there is no red light, the phosphorylated OmpR then turn on PompC, causing LacI to be expressed and repress Plac to translate the genes downstream. Nctu_formosa_pred.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1282
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1296
  • 1000
    COMPATIBLE WITH RFC[1000]