Difference between revisions of "Part:BBa K1132014:Design"
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===Design Notes=== | ===Design Notes=== | ||
The monomeric RFP is a Red Fluorescent Protein with an excitation peak at 584 nm and an emission peak at 607 nm. The RFP is positively regulated by OmpR-controlled promoter. Phosphorylated OmpR binds to the operator sites and activates transcription of RFP. | The monomeric RFP is a Red Fluorescent Protein with an excitation peak at 584 nm and an emission peak at 607 nm. The RFP is positively regulated by OmpR-controlled promoter. Phosphorylated OmpR binds to the operator sites and activates transcription of RFP. | ||
| − | This is a test Biobrick for the Red light responsive system (Part:BBa_K1132013 and Part:BBa_K1132015) with the RFP protein as output. EnvZ phosphorylates OmpR to OmpR-P that activate the promoter. It must be used in E.coli deficient in wild-type EnvZ. | + | <br><br>This is a test Biobrick for the Red light responsive system (Part:BBa_K1132013 and Part:BBa_K1132015) with the RFP protein as output. EnvZ phosphorylates OmpR to OmpR-P that activate the promoter. It must be used in E.coli deficient in wild-type EnvZ. |
Latest revision as of 09:09, 20 September 2013
Promoter (OmpR, positive) followed by RFP protein generator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 692
Illegal AgeI site found at 804 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The monomeric RFP is a Red Fluorescent Protein with an excitation peak at 584 nm and an emission peak at 607 nm. The RFP is positively regulated by OmpR-controlled promoter. Phosphorylated OmpR binds to the operator sites and activates transcription of RFP.
This is a test Biobrick for the Red light responsive system (Part:BBa_K1132013 and Part:BBa_K1132015) with the RFP protein as output. EnvZ phosphorylates OmpR to OmpR-P that activate the promoter. It must be used in E.coli deficient in wild-type EnvZ.
Source
later