Difference between revisions of "Part:BBa K1062000"
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==='''Usage and Biology'''=== | ==='''Usage and Biology'''=== | ||
In both of our projects, we used gRNAs and dCas9 as: | In both of our projects, we used gRNAs and dCas9 as: | ||
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1) To specifically target and repress the "virulence gene" (we used flourescent proteins as a proof of concept) in our conjugation project | 1) To specifically target and repress the "virulence gene" (we used flourescent proteins as a proof of concept) in our conjugation project | ||
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2) Improve past circuits with better decision making by using dCas9 instead of traditional repressors. | 2) Improve past circuits with better decision making by using dCas9 instead of traditional repressors. | ||
Revision as of 06:06, 20 September 2013
Guide RNA (gRNA) target for RFP
This part is a guide RNA (gRNA) that targets RFP, when bound to Cas9. It also contains a cleavage site recognized by the Csy4 enzyme which allows it to be cleaved from neighboring DNA/RNA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
In both of our projects, we used gRNAs and dCas9 as:
1) To specifically target and repress the "virulence gene" (we used flourescent proteins as a proof of concept) in our conjugation project
2) Improve past circuits with better decision making by using dCas9 instead of traditional repressors.
Conjugation Project
Synthetic Circuit
