Difference between revisions of "Part:BBa K1062002"
(→Usage and Biology) |
|||
| Line 11: | Line 11: | ||
==='''Usage and Biology'''=== | ==='''Usage and Biology'''=== | ||
In both of our projects, we used gRNAs and dCas9 as: | In both of our projects, we used gRNAs and dCas9 as: | ||
| + | |||
1) To specifically target and repress the "virulence gene" (we used flourescent proteins as a proof of concept) in our conjugation project However, the gRNA for XylE is not used in the conjugation project since we did not use any of the pigments in this project. | 1) To specifically target and repress the "virulence gene" (we used flourescent proteins as a proof of concept) in our conjugation project However, the gRNA for XylE is not used in the conjugation project since we did not use any of the pigments in this project. | ||
| + | |||
2) Improve past circuits with better decision making by using dCas9 instead of traditional repressors. | 2) Improve past circuits with better decision making by using dCas9 instead of traditional repressors. | ||
Revision as of 06:06, 20 September 2013
Guide RNA (gRNA) target for XylE (middle of gene)
This part is a guide RNA (gRNA) that targets the middle of the XylE gene, when bound to Cas9.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
In both of our projects, we used gRNAs and dCas9 as:
1) To specifically target and repress the "virulence gene" (we used flourescent proteins as a proof of concept) in our conjugation project However, the gRNA for XylE is not used in the conjugation project since we did not use any of the pigments in this project.
2) Improve past circuits with better decision making by using dCas9 instead of traditional repressors.
Conjugation Project
The XylE (middle) gRNA was not used in the Conjugation Project
Synthetic Circuit
