Difference between revisions of "Part:BBa K1062003"
(→‎Conjugation Project)
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=='''Conjugation Project'''==
 
=='''Conjugation Project'''==
  
−
'''The XylE (middle) gRNA was not used in the Conjugation Project'''
+
'''The XylE (end) gRNA was not used in the Conjugation Project'''
  
 
=='''Synthetic Circuit'''==
 
=='''Synthetic Circuit'''==

Revision as of 06:06, 20 September 2013

Guide RNA (gRNA) target for XylE (end of gene)

This part is a guide RNA (gRNA) that targets the end of the XylE gRNA, when bound to Cas9.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

In both of our projects, we used gRNAs and dCas9 as: 1) To specifically target and repress the "virulence gene" (we used flourescent proteins as a proof of concept) in our conjugation project However, the gRNA for XylE is not used in the conjugation project since we did not use any of the pigments in this project. 2) Improve past circuits with better decision making by using dCas9 instead of traditional repressors.

Conjugation Project

The XylE (end) gRNA was not used in the Conjugation Project

Synthetic Circuit

Synthetic Circuit.jpg