Difference between revisions of "Part:BBa K1062001"
| Line 12: | Line 12: | ||
==='''Usage and Biology'''=== | ==='''Usage and Biology'''=== | ||
In both of our projects, we used gRNAs and dCas9 as: | In both of our projects, we used gRNAs and dCas9 as: | ||
| − | 1) To specifically target and repress the "virulence gene" (we used flourescent proteins as a proof of concept) in our conjugation project | + | 1) To specifically target and repress the "virulence gene" (we used flourescent proteins as a proof of concept) in our conjugation project. However, the gRNA for GFP is not used in the conjugation project since the green fluorescent protein is to represent the donor strain of the project |
2) Improve past circuits with better decision making by using dCas9 instead of traditional repressors. | 2) Improve past circuits with better decision making by using dCas9 instead of traditional repressors. | ||
| − | |||
=='''Conjugation Project'''== | =='''Conjugation Project'''== | ||
Revision as of 06:04, 20 September 2013
Guide RNA (gRNA) target for GFP
This part is a guide RNA (gRNA) that targets GFP, when bound to Cas9. It also contains a cleavage site recognized by the Csy4 enzyme which allows it to be cleaved from neighboring DNA/RNA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
In both of our projects, we used gRNAs and dCas9 as: 1) To specifically target and repress the "virulence gene" (we used flourescent proteins as a proof of concept) in our conjugation project. However, the gRNA for GFP is not used in the conjugation project since the green fluorescent protein is to represent the donor strain of the project 2) Improve past circuits with better decision making by using dCas9 instead of traditional repressors.
Conjugation Project
The GFP gRNA was not used in the Conjugation Project
Synthetic Circuit
