Difference between revisions of "Part:BBa K1062002"

Revision as of 06:02, 20 September 2013

Guide RNA (gRNA) target for XylE (middle of gene)

This part is a guide RNA (gRNA) that targets the middle of the XylE gene, when bound to Cas9.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Usage and Biology

In both of our projects, we used gRNAs and dCas9 as: 1) To specifically target and repress the "virulence gene" (we used flourescent proteins as a proof of concept) in our conjugation project 2) Improve past circuits with better decision making by using dCas9 instead of traditional repressors. However, the gRNA for GFP is not used in the conjugation project since the green fluorescent protein is to represent the donor strain of the project.

Conjugation Project

The GFP gRNA was not used in the Conjugation Project

@@ Line 9: / Line 9: @@
 <partinfo>BBa_K1062002 SequenceAndFeatures</partinfo>
-<!-- Add more about the biology of this part here
+==='''Usage and Biology'''===
-===Usage and Biology===
+In both of our projects, we used gRNAs and dCas9 as:
+) To specifically target and repress the "virulence gene" (we used flourescent proteins as a proof of concept) in our conjugation project
+) Improve past circuits with better decision making by using dCas9 instead of traditional repressors.
+However, the gRNA for GFP is not used in the conjugation project since the green fluorescent protein is to represent the donor strain of the project.
+=='''Conjugation Project'''==
+'''The GFP gRNA was not used in the Conjugation Project'''
+=='''Synthetic Circuit'''==
+[[File:Synthetic Circuit.jpg]]
 <!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
 <partinfo>BBa_K1062002 parameters</partinfo>
 <!-- -->