Difference between revisions of "Part:BBa K1076005:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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We chosed the ORF of this gene to be expressed into Shewanella putrefaciens making a super expression since the bacteria already have one copy of this gene in its genome. We made the primers pair with EcoR1 (5') and Pst1 (3') ends in order to clone into pSB1C3. Further subclonning and expression should be done within pSB3C5. Should also be assembled with promoter, RBS and terminator.
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We chosed the ORF of this gene to be expressed into Shewanella putrefaciens making a super expression since the bacteria already have one copy of this gene in its genome. We made the primers pair with EcoR1 (5') and Pst1 (3') ends in order to clone into pSB1C3. Further subclonning and expression should be done within pSB3C5-152001. Should also be assembled with promoter, RBS and terminator.
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===Source===
 
===Source===

Revision as of 23:40, 19 September 2013


FadL, Long-chain fatty acid outer membrane transporte


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 716
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We chosed the ORF of this gene to be expressed into Shewanella putrefaciens making a super expression since the bacteria already have one copy of this gene in its genome. We made the primers pair with EcoR1 (5') and Pst1 (3') ends in order to clone into pSB1C3. Further subclonning and expression should be done within pSB3C5-152001. Should also be assembled with promoter, RBS and terminator.

Source

From Genomic DNA from Shewanella putrefaciens

References