Difference between revisions of "Part:BBa K1211001"

Revision as of 23:00, 19 September 2013

Clostridum propionicum propionate CoA transferase

This is the DNA coding for the enzyme propionate CoA transferase from Clostridum propionicum. The enzyme normally catalyzes the reaction: acetyl-CoA + propanoate <--> acetate + propanoyl-CoA. This mutated sequence has four point mutations and one amino acid substitution (compared to the wild type enzyme) in order to produce (D)-Lactyl-CoA.
We used this enzyme as a the first enzyme in the pathway to create PLA or Polylactic acid. To read about our project [http://2013.igem.org/Team:Yale/Project_Overview click here]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K1211001 short</partinfo>
-This is the DNA coding for the enzyme propionate CoA transferase from Clostridum propionicum.  The enzyme normally catalyzes the reaction: acetyl-CoA + propanoate <--> acetate + propanoyl-CoA.  This mutated sequence has four point mutations and one amino acid substitution (compared to the wild type enzyme) in order to produce (D)-Lactyl-CoA.
+This is the DNA coding for the enzyme propionate CoA transferase from Clostridum propionicum.  The enzyme normally catalyzes the reaction: acetyl-CoA + propanoate <--> acetate + propanoyl-CoA.  This mutated sequence has four point mutations and one amino acid substitution (compared to the wild type enzyme) in order to produce (D)-Lactyl-CoA.  <br>
+We used this enzyme as a the first enzyme in the pathway to create PLA or Polylactic acid.
+To read about our project [http://2013.igem.org/Team:Yale/Project_Overview  click here] <br>
 [[File:PLA pathway2.jpg|600px]]
 <!-- Add more about the biology of this part here