Difference between revisions of "Part:BBa K1154000"

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<partinfo>BBa_K1154000 short</partinfo>
 
<partinfo>BBa_K1154000 short</partinfo>
  
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Synthetic DNA optimized for expression in Saccharomyces cerevisie. The construct harbors a yeast HIS3 gene, which encodes imidazoleglycerol-phosphate dehydratase, under control of the mating pheromone-induced FUS1 promoter. It also harbors a synthetic gene encoding human lactate dehydrogenase (LDHA) under control of the constitutive TEF2 promoter.
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Synthetic DNA optimized for expression in Saccharomyces cerevisie. The construct harbors a yeast HIS3 gene, which encodes imidazoleglycerol-phosphate dehydratase, under control of the mating pheromone-induced FUS1 promoter. It also harbors a gene encoding human lactate dehydrogenase (LDHA) under control of the constitutive TEF2 promoter.
  
 
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Revision as of 11:34, 19 September 2013

Mating pheromone-induced IGPD and constitutive LDH expression in Yeast

Synthetic DNA optimized for expression in Saccharomyces cerevisie. The construct harbors a yeast HIS3 gene, which encodes imidazoleglycerol-phosphate dehydratase, under control of the mating pheromone-induced FUS1 promoter. It also harbors a gene encoding human lactate dehydrogenase (LDHA) under control of the constitutive TEF2 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 763
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]