Difference between revisions of "Part:BBa K1025003"
| Line 11: | Line 11: | ||
<!-- --> | <!-- --> | ||
We measured the mutation increase induced by our mut part with the protocol described in our note from. To be brief, by measuring the reversion of rifampinresistance caused by mutation in genome, we accurately determined the mutation rate increase to be as big as four times compared with negative control (either bacteria without mutD or without arabinose input). The data is shown below. | We measured the mutation increase induced by our mut part with the protocol described in our note from. To be brief, by measuring the reversion of rifampinresistance caused by mutation in genome, we accurately determined the mutation rate increase to be as big as four times compared with negative control (either bacteria without mutD or without arabinose input). The data is shown below. | ||
| − | [[File:mutD-1.png| | + | [[File:mutD-1.png|450px|thumb|left|alt text]] |
| − | [[File:mutD-2.png| | + | [[File:mutD-2.png|450px|thumb|left|alt text]] |
<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K1025006 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1025006 SequenceAndFeatures</partinfo> | ||
Revision as of 14:05, 18 September 2013
Thu-E Mutation Part
iGEM mut part is a plasmid constructed by 2013 iGEM Tsinghua-E team which is used for genome level in vivo high-diversity library construction.
Usage and Biology
In this vector, highly error-prone dnaQ mutant, mutD1 was cloned downstream of araBAD promoter so that we can control the mutation intensity of the target genome by the concentration of araBAD promoter’s inducer, L-arabinose in a strict manner. And the GFP downstream mutD was to test whether the mutD can be translated.
Functional Parameters
We measured the mutation increase induced by our mut part with the protocol described in our note from. To be brief, by measuring the reversion of rifampinresistance caused by mutation in genome, we accurately determined the mutation rate increase to be as big as four times compared with negative control (either bacteria without mutD or without arabinose input). The data is shown below.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1750