Difference between revisions of "Part:BBa K1150032"
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==Introduction== | ==Introduction== | ||
| − | This part contains the protein COP1 fused to the Methyl-Tranferase G9a. Together with the UV-light switch Part I, you will be able to repress virtually any desired gene indicated by a short UV-light pulse. COP1 is a protein that was first described in the model organism ''Arabidopsis thaliana''.It will bind to the UV-light receptor UVR8 after illumination. Thereby recruiting G9a to its target locus. Subsequently the local DNA-expression will be repressed by DNA-methylation | + | This part contains the protein COP1 fused to the Methyl-Tranferase G9a. Together with the UV-light switch Part I, you will be able to repress virtually any desired gene indicated by a short UV-light pulse. COP1 is a protein that was first described in the model organism ''Arabidopsis thaliana''. It will bind to the UV-light receptor UVR8 after illumination. Thereby recruiting G9a to its target locus. Subsequently the local DNA-expression will be repressed by DNA-methylation |
== References == | == References == | ||
Revision as of 13:41, 18 September 2013
uniCAS UV Light Switch Part II - Histone Modifier
Introduction
This part contains the protein COP1 fused to the Methyl-Tranferase G9a. Together with the UV-light switch Part I, you will be able to repress virtually any desired gene indicated by a short UV-light pulse. COP1 is a protein that was first described in the model organism Arabidopsis thaliana. It will bind to the UV-light receptor UVR8 after illumination. Thereby recruiting G9a to its target locus. Subsequently the local DNA-expression will be repressed by DNA-methylation
References
Muller, K.; Engesser, R.; Schulz, S.; Steinberg, T.; Tomakidi, P.; Weber, C. C. et al. (Nucleid Acids Research 2013): Multi-chromatic control of mammalian gene expression and signaling
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 576
Illegal BglII site found at 2231 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1373
Illegal BsaI.rc site found at 1450
Illegal SapI.rc site found at 962