Difference between revisions of "Part:BBa K1150025"
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Our construct contains the standardized version of Cas9 linked to PIF6. This device is one of two parts that you need to enable red-light induced gene targeting. Cotransfection of a PhyB+effector device, and target RNA allows you to recruit any desired effector to your desired Gene locus. Illumination with red light (660nm) will start the interaction and illumination with far red light (740nm) will stop the interaction. | Our construct contains the standardized version of Cas9 linked to PIF6. This device is one of two parts that you need to enable red-light induced gene targeting. Cotransfection of a PhyB+effector device, and target RNA allows you to recruit any desired effector to your desired Gene locus. Illumination with red light (660nm) will start the interaction and illumination with far red light (740nm) will stop the interaction. | ||
The Cas9-version used here is human codon optimized and the mutated version, unable to cleave DNA. PIF6 is human codon-ptimized. | The Cas9-version used here is human codon optimized and the mutated version, unable to cleave DNA. PIF6 is human codon-ptimized. | ||
| + | =References:= | ||
| + | Muller, K.; Engesser, R.; Metzger, S.; Schulz, S.; Kampf, M. M.; Busacker, M. et al. ((Nucleid Acid Research; 2013): A red/far-red light-responsive bi-stable toggle switch to control gene expression in mammalian cells. | ||
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Revision as of 13:17, 18 September 2013
uniCAS Red Light Switch Part I - Stimulator
Introduction
Our construct contains the standardized version of Cas9 linked to PIF6. This device is one of two parts that you need to enable red-light induced gene targeting. Cotransfection of a PhyB+effector device, and target RNA allows you to recruit any desired effector to your desired Gene locus. Illumination with red light (660nm) will start the interaction and illumination with far red light (740nm) will stop the interaction. The Cas9-version used here is human codon optimized and the mutated version, unable to cleave DNA. PIF6 is human codon-ptimized.
References:
Muller, K.; Engesser, R.; Metzger, S.; Schulz, S.; Kampf, M. M.; Busacker, M. et al. ((Nucleid Acid Research; 2013): A red/far-red light-responsive bi-stable toggle switch to control gene expression in mammalian cells.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 576
Illegal BglII site found at 900 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]