Difference between revisions of "Part:BBa K1017726:Design"
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===References=== | ===References=== | ||
| − | Anselm Levskaya, Aaron A. Chevalier, Jeffrey J. | + | Anselm Levskaya, Aaron A. Chevalier, Jeffrey J. Tabor, Zachary Booth Simpson, Laura A. Lavery, Matthew Levy, Eric A. Davidson, Alexander Scouras, Andrew D. Ellington, Edward M. Marcotte, Christopher A. Voigt.(2005). Engineering Escherichia coli to see light, Nature,438:441 |
Elva J. H. Robinson, Duncan E. Jackson,Mike Holcombe, Francis L. W. Ratnieks.(2005). ‘No entry’ signal in ant foraging, Nature,438:442 | Elva J. H. Robinson, Duncan E. Jackson,Mike Holcombe, Francis L. W. Ratnieks.(2005). ‘No entry’ signal in ant foraging, Nature,438:442 | ||
Latest revision as of 13:07, 18 September 2013
Pcons+B0030+pcya+B0032+ho1+B0030
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 833
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 386
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is which we use to change heme into phycocyanobilin(PCB). We use the two different RBS to prevent Homologous recombination, which will cause the mutation of this part.
Source
assemble parts form iGEM distribution I115008, I115009, I115010
References
Anselm Levskaya, Aaron A. Chevalier, Jeffrey J. Tabor, Zachary Booth Simpson, Laura A. Lavery, Matthew Levy, Eric A. Davidson, Alexander Scouras, Andrew D. Ellington, Edward M. Marcotte, Christopher A. Voigt.(2005). Engineering Escherichia coli to see light, Nature,438:441
Elva J. H. Robinson, Duncan E. Jackson,Mike Holcombe, Francis L. W. Ratnieks.(2005). ‘No entry’ signal in ant foraging, Nature,438:442