Difference between revisions of "Part:BBa K1088017:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
−
The GTG start codon was changed into ATG
+
The constitutive promoter was used along with a strong RBS to ensure a constant high level of AraC protein.
−
 
+
A strong terminator was used to prevent transcription of downstream genes.
−
 
+
  
 
===Source===
 
===Source===

Revision as of 11:22, 18 September 2013


AraC device (constitutive promoter, RBS and terminator)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The constitutive promoter was used along with a strong RBS to ensure a constant high level of AraC protein. A strong terminator was used to prevent transcription of downstream genes.

Source

>gnl|ECOLI|EG10525 lacI "PD00763" (complement(366734..365652)) Escherichia coli K-12 substr. MG1655

References