Difference between revisions of "Part:BBa K1150016"

Revision as of 22:37, 17 September 2013

HA tag

The human influenza hemagglutinin (HA)–tag enables the detection, isolation and purification of proteins.
Engineered into recombinant proteins, it does not seem to interfere with its bioactivity or biodistribution.
It is derived from a surface glycoprotein of the human virus. The tag consists only of the amino acids 98-106 of the original protein.

The HA-tag was used mainly for protein detection. After blotting our SDS-PAGE gels on PVDF membranes, anti-HA-antibodies were used to target our recombinant proteins. Secondary antibodies furnished proof of our proteins.

Figure 1: Proteins with HA-tag were detected with anti-HA-antibody on a Western Blot.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1150016 short</partinfo>
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+The human influenza hemagglutinin (HA)–tag enables the detection, isolation and purification of proteins. <br> Engineered into recombinant proteins, it does not seem to interfere with its bioactivity or biodistribution. <br> It is derived from a surface glycoprotein of the human virus. The tag consists only of the amino acids 98-106 of the original protein. <br><br>
+The HA-tag was used mainly for protein detection. After blotting our SDS-PAGE gels on PVDF membranes, anti-HA-antibodies were used to target our recombinant proteins. Secondary antibodies furnished proof of our proteins.
+https://static.igem.org/mediawiki/2013/4/43/Ha-tag_registry_freiburg_13.PNG
+<br>
+'''Figure 1:''' Proteins with HA-tag were detected with anti-HA-antibody on a Western Blot.
 <!-- Add more about the biology of this part here