Difference between revisions of "Part:BBa K1039016"

 
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HPdO Helper Plasmid contains the M13 viral genome but is lacking the f1 origin and packaging sequence. It contains the p15A origin of replication and a kanamycin resistance gene (Lin et al., 2011).
  
 
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Revision as of 17:25, 17 September 2013

HPdO Helper Plasmid

HPdO Helper Plasmid contains the M13 viral genome but is lacking the f1 origin and packaging sequence. It contains the p15A origin of replication and a kanamycin resistance gene (Lin et al., 2011).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 8168
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5916
    Illegal PstI site found at 8168
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2622
    Illegal XhoI site found at 8010
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 8168
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 8168
    Illegal AgeI site found at 5956
    Illegal AgeI site found at 6280
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 8173
    Illegal BsaI.rc site found at 6985