Difference between revisions of "Part:BBa J45993:Design"
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===Source=== | ===Source=== | ||
| − | This part was PCRed out of E. coli's genome. | + | This part was PCRed out of E. coli's genome with the primers listed in the Design Notes section. |
===References=== | ===References=== | ||
Revision as of 20:37, 11 July 2006
Minimal stationary phase osmY promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Truncated Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GGCT TAT GTT TTC GCT GAT ATC - 3'
Total Length: 49 bp
Annealing Length: 21 bp
GC Content: 38.1%
Melting Temperature: 49.5 degrees C
hairpin deltaG: -2.66 kcal/mol
self dimer deltaG: -99.97 kcal/mol
Reverse Primer: 5'-GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TGT TAA ATA TAG ATC ACA ATT TTG- 3'
Total Length: 54 bp
Annealing Length: 25 bp
GC Content: 20.0%
Melting Temperature: 46.4 degrees C
hairpin deltaG: -2.86 kcal/mol
self dimer deltaG: -99.44 kcal/mol
heterodimer deltaG with Conserved Forward Primer: -99.97 kcal/mol
Source
This part was PCRed out of E. coli's genome with the primers listed in the Design Notes section.