Difference between revisions of "Part:BBa K1113600"
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<partinfo>BBa_K1113600 short</partinfo> | <partinfo>BBa_K1113600 short</partinfo> | ||
| − | GFP | + | This part consist in the superfolder GFP (BBa_I746916) attached to two targeting sequences: The large subunit of the ribulose-1,5-bisphosphate carboxylase/oxygenase enzyme which deliver into the carboxysome and the ssrA degradation tag which is a targeting sequence for degradation by the protease ClpXP. This construct is used as a reporter for the correct assembly of the Carboxysome, it works with the competition of the two targeting sequence, in the case of a correct assembly of the carboxysome the microcompartment will “protect” the fluorescens, in any other case this will be degraded by the protease. |
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Latest revision as of 15:39, 17 September 2013
GFP with a targeting sequence for delivery into the Carboxysome and a ssrA degradation tag.
This part consist in the superfolder GFP (BBa_I746916) attached to two targeting sequences: The large subunit of the ribulose-1,5-bisphosphate carboxylase/oxygenase enzyme which deliver into the carboxysome and the ssrA degradation tag which is a targeting sequence for degradation by the protease ClpXP. This construct is used as a reporter for the correct assembly of the Carboxysome, it works with the competition of the two targeting sequence, in the case of a correct assembly of the carboxysome the microcompartment will “protect” the fluorescens, in any other case this will be degraded by the protease.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]