Difference between revisions of "Part:BBa C0072:Design"

(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
−
wild type dimeric repressor from enterobacteriophage p22 with dissociation constant of 2.5*10^-12.
+
wild type dimeric repressor from enterobacteriophage p22 with dissociation constant of 2.5*10^-12.<br>
−
sequence was translated with the E.Coli K12 codon usage table, and nucleotides 95-97 was changed from AAT to AAC to avoid the cut site GAATTC (94-99).
+
sequence was translated with the E.Coli K12 codon usage table, and nucleotides 95-97 was changed from AAT to AAC to avoid the cut site GAATTC (94-99).<br><br>
  
−
Identification of Functionally Important Residues in the DNA Binding Region of the MNT Repressor
+
Identification of Functionally Important Residues in the DNA Binding Region of the MNT Repressor<br>
−
Knight, KL; Sauer, RT
+
Knight, KL; Sauer, RT<br>
−
The Journal of Biological Chemistry, 264(23):13706-13710, 1989
+
The Journal of Biological Chemistry, 264(23):13706-13710, 1989<br>
 
(PMID: 2668272)
 
(PMID: 2668272)
  

Revision as of 19:28, 11 July 2006


mnt repressor (strong) from Salmonella phage P22 (+LVA)


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

wild type dimeric repressor from enterobacteriophage p22 with dissociation constant of 2.5*10^-12.
sequence was translated with the E.Coli K12 codon usage table, and nucleotides 95-97 was changed from AAT to AAC to avoid the cut site GAATTC (94-99).

Identification of Functionally Important Residues in the DNA Binding Region of the MNT Repressor
Knight, KL; Sauer, RT
The Journal of Biological Chemistry, 264(23):13706-13710, 1989
(PMID: 2668272)

Source

enterobacteriophage p22

References