Difference between revisions of "Part:BBa K1041001"

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===BLAST Analysis===
 
===BLAST Analysis===
 
The data we recieved back from the sequencing company was aligned using BLAST with the expected DNA sequence.
 
The data we recieved back from the sequencing company was aligned using BLAST with the expected DNA sequence.
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[[image:3a Fwd.JPG]]
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[[image:3a Fwd.JPG|thumb|sequenced Bba_K1041000 DNA with Forward Primer aligned with expected DNA Sequence]]

Revision as of 11:51, 17 September 2013

Neomycin Resistance Coding Device + AntG promoter

Team NRP-UEA_Norwich 2013 designed this part to contain the promoter sequence AntG, where the unique sigma factor AntA binds to activate transcription of the neomycin resistance gene. There is a Nde1 resticition site between the promoter and gene to allow either to be removed and exchanged for a different promoter or gene. This facilitated cloning of the biobrick Bba_K1041002

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 757
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 606
    Illegal SapI.rc site found at 816


Characterisation

Characterisation of this biobrick involved sequencing, restriction digests and BLAST analysis.

Sequencing

The biobrick was sent off to a company for sequencing.

K1041000 sequencing data part 1
K1041000 sequencing data part 2
K1041000 sequencing data part 3










BLAST Analysis

The data we recieved back from the sequencing company was aligned using BLAST with the expected DNA sequence.

sequenced Bba_K1041000 DNA with Forward Primer aligned with expected DNA Sequence