Difference between revisions of "Part:BBa K1017101:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | Since the transcription factor of PompC, OmpR, is phosphorylated in dark and non phosphorylated in red light, we used LacI and Plac downstream PompC to reverse the original function of PompC. By this way, we can express the genes downstream in red light and block them in dark, making this biobrick a red light-induced promoter. | |
Revision as of 11:19, 17 September 2013
Pred ( PompC + B0032 + LacI + J61048 + Plac)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1282
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1296
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Since the transcription factor of PompC, OmpR, is phosphorylated in dark and non phosphorylated in red light, we used LacI and Plac downstream PompC to reverse the original function of PompC. By this way, we can express the genes downstream in red light and block them in dark, making this biobrick a red light-induced promoter.
Source
assemble parts form iGEM distribution