Difference between revisions of "Part:BBa K1041001"
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Team NRP-UEA_Norwich 2013 designed this part to contain the promoter sequence AntG, where the unique sigma factor AntA binds to activate transcription of the neomycin resistance gene. There is a Nde1 resticition site between the promoter and gene to allow either to be removed and exchanged for a different promoter or gene. This faciliated cloning of the biobrick [[Bba_K1041002]]   
 
Team NRP-UEA_Norwich 2013 designed this part to contain the promoter sequence AntG, where the unique sigma factor AntA binds to activate transcription of the neomycin resistance gene. There is a Nde1 resticition site between the promoter and gene to allow either to be removed and exchanged for a different promoter or gene. This faciliated cloning of the biobrick [[Bba_K1041002]]   
  
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===User Reviews===
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This part contains the promoter sequence AntG, where the unique sigma factor AntA binds to activate transcription of the neomycin resistance gene. There is a Nde1 resticition site between the promoter and gene to allow either to be removed and exchanged for a different promoter or gene. 
 
  
 
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<partinfo>BBa_K1041001 parameters</partinfo>
 
<partinfo>BBa_K1041001 parameters</partinfo>
 
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==Characterisation==
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Characterisation of this biobrick involved sequencing, restriction digests and BLAST analysis.
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===Sequencing===
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The biobrick was sent off to a company for sequencing.
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[[image:3a vf2 0-540.JPG|thumb|left|K1041000 sequencing data part 1]]
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[[image:3a vf2 440-860.JPG|thumb|left|K1041000 sequencing data part 2 ]]
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[[image:3a vf2 860-.JPG|thumb|left|K1041000 sequencing data part 3]]
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===BLAST Analysis===
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The data we recieved back from the sequencing company was aligned using BLAST with the expected DNA sequence ''fig.2''

Revision as of 11:13, 17 September 2013

Neomycin Resistance Coding Device + AntG promoter

Team NRP-UEA_Norwich 2013 designed this part to contain the promoter sequence AntG, where the unique sigma factor AntA binds to activate transcription of the neomycin resistance gene. There is a Nde1 resticition site between the promoter and gene to allow either to be removed and exchanged for a different promoter or gene. This faciliated cloning of the biobrick Bba_K1041002


UNIQ79b70e28938d0195-partinfo-00000001-QINU UNIQ79b70e28938d0195-partinfo-00000002-QINU


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 757
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 606
    Illegal SapI.rc site found at 816


Characterisation

Characterisation of this biobrick involved sequencing, restriction digests and BLAST analysis.

Sequencing

The biobrick was sent off to a company for sequencing.

K1041000 sequencing data part 1
K1041000 sequencing data part 2
K1041000 sequencing data part 3










BLAST Analysis

The data we recieved back from the sequencing company was aligned using BLAST with the expected DNA sequence fig.2