Difference between revisions of "Part:BBa K1116001"
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<partinfo>BBa_K1116001 short</partinfo> | <partinfo>BBa_K1116001 short</partinfo> | ||
| − | It was designed as a intermediate product to construct BBa_K1116003 in our project. | + | It was designed as a intermediate product to construct BBa_K1116003 in our project. .We use this part to construct K1116003.However,K1116003 cannot be assembled in pSB1C3 because target gene has ECOR1 restriction site .We have tested K1116003 .The results can be seen in K1116002's experience. [[1]] So the plasmid K1116001 works because K1116003 have been tested. |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 05:16, 17 September 2013
LacI with miR-FF5 and miR-FF3 target
It was designed as a intermediate product to construct BBa_K1116003 in our project. .We use this part to construct K1116003.However,K1116003 cannot be assembled in pSB1C3 because target gene has ECOR1 restriction site .We have tested K1116003 .The results can be seen in K1116002's experience. 1 So the plasmid K1116001 works because K1116003 have been tested.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1120
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]