Difference between revisions of "Part:BBa K1116000"
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<partinfo>BBa_K1116000 short</partinfo> | <partinfo>BBa_K1116000 short</partinfo> | ||
| − | This part is a LacI coding sequence with 4 miR-21 and 4 miR-FF3 target sites so it can be regulated with micro-RNA 21 and FF3.[[https://parts.igem.org/Part:BBa_K1116002:Experience]] | + | This part is a LacI coding sequence with 4 miR-21 and 4 miR-FF3 target sites so it can be regulated with micro-RNA 21 and FF3.We use this part to construct K1116002.However,K1116002 cannot be assembled in pSB1C3 because target gene has ECOR1 restriction site .We have |
| + | tested K1116002 .The results can be seen in K1116002's experience. [[https://parts.igem.org/Part:BBa_K1116002:Experience]] | ||
| + | So the plasmid K1116000 works because K1116002 have been tested. | ||
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Revision as of 05:14, 17 September 2013
LacI with miR-21 and miR-FF3 target
This part is a LacI coding sequence with 4 miR-21 and 4 miR-FF3 target sites so it can be regulated with micro-RNA 21 and FF3.We use this part to construct K1116002.However,K1116002 cannot be assembled in pSB1C3 because target gene has ECOR1 restriction site .We have tested K1116002 .The results can be seen in K1116002's experience. [[1]] So the plasmid K1116000 works because K1116002 have been tested.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1120
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]