Difference between revisions of "An Introduction to BioBricks"
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Physical parts in the DNA Repository have been designed to be assembled into systems using normal cloning techniques based on [[Assembly:Restriction enzymes|restriction enzymes]], purification, ligation, and transformation - with a twist: BioBrick parts are [[Assembly:Composable|composable]]. The result of assembling two parts is a new part that may be used in future assemblies. | Physical parts in the DNA Repository have been designed to be assembled into systems using normal cloning techniques based on [[Assembly:Restriction enzymes|restriction enzymes]], purification, ligation, and transformation - with a twist: BioBrick parts are [[Assembly:Composable|composable]]. The result of assembling two parts is a new part that may be used in future assemblies. | ||
| − | + | ;Standard Assembly | |
| − | + | :The classical method of BioBrick assembly, done at the lab bench. | |
| − | Insert pic of std assembly | + | :Insert pic of std assembly |
| − | + | ;Automated Assembly | |
| − | + | :Automated assembly, currently in late stage development at iGEM labs. | |
| − | + | :Insert pic of auto assembly | |
| − | Insert pic of auto assembly | + | |
==How do I make a BioBrick?== | ==How do I make a BioBrick?== | ||
Revision as of 21:42, 7 July 2006
Contents
What is a BioBrick?
The BioBrick logic family is a set of interchangable components, designed with a view to building biological systems in living cells. The members of this family are designed to be compatible, composible, interchangeable and independent; in this way a new generation of biological engineers are building novel devices much in the way their electronically-orientated collegues might piece together transistors, resistors and capacitors.
A BioBrick represents a unique sequence of DNA; it might be a gene that codes a protein or a switch triggered by an external input, it also has a unique name. Whatever its function, a biobrick comes packaged in a plasmid, ready to be assembled into useful devices & systems.
Building BioBrick Systems
Physical parts in the DNA Repository have been designed to be assembled into systems using normal cloning techniques based on restriction enzymes, purification, ligation, and transformation - with a twist: BioBrick parts are composable. The result of assembling two parts is a new part that may be used in future assemblies.
- Standard Assembly
- The classical method of BioBrick assembly, done at the lab bench.
- Insert pic of std assembly
- Automated Assembly
- Automated assembly, currently in late stage development at iGEM labs.
- Insert pic of auto assembly
How do I make a BioBrick?
At the bench
Pic of biobrick flanked by coding seq
Methods:
- Synthesis
- PCR
Adding the part to the registry
This protocol is quick and easy. A comprehensive guide can be found here