Difference between revisions of "An Introduction to BioBricks"

(→‎Standard Assembly)
Line 10: Line 10:
  
 
Insert pic of std assembly
 
Insert pic of std assembly
 +
 +
==How do I make a BioBrick?==
 +
===At the bench===
 +
 +
Pic of biobrick flanked by coding seq
 +
 +
Methods:
 +
*Synthesis
 +
*PCR
 +
 +
===Adding the part to the registry===
 +
comprehensive guide can be found [[Basic Biobrick, example 1|here]]

Revision as of 19:25, 7 July 2006

What is a BioBrick?

The BioBrick logic family is a set of interchangable components, designed with a view to building biological systems in living cells. The members of this family are designed to be compatible, composible, interchangeable and independent; in this way a new generation of biological engineers are building novel devices much in the way their electronically-orientated collegues might piece together transistors, resistors and capacitors.

A BioBrick represents a unique sequence of DNA; it might be a gene that codes a protein or a switch triggered by an external input, it also has a unique name. Whatever its function, a biobrick comes packaged in a plasmid, ready to be assembled into useful devices & systems.

Building BioBrick Systems

Physical parts in the DNA Repository have been designed to be assembled into systems using normal cloning techniques based on restriction enzymes, purification, ligation, and transformation - with a twist: BioBrick parts are composable. The result of assembling two parts is a new part that may be used in future assemblies.

Standard AssemblyThe classical method of BioBrick assembly, done at the lab bench

Insert pic of std assembly

How do I make a BioBrick?

At the bench

Pic of biobrick flanked by coding seq

Methods:

  • Synthesis
  • PCR

Adding the part to the registry

comprehensive guide can be found here