Difference between revisions of "Part:BBa K1139019"

 
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<partinfo>BBa_K1139019 short</partinfo>
 
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M13 is a filamentous phage that infects only F+ strains of E. coli, which does not kill the host cell.
 
M13 is a filamentous phage that infects only F+ strains of E. coli, which does not kill the host cell.
 
This biobrick is derived from M13mp18 phage vector, amplified by PCR. It includes 11 ORFs, M13 origin, a packaging sequence and Lac promoter. The promoter on the upstream of g2(gene 2) is deleted.The capsid is formed only when g2p(gene 2 protein) is expressed.
 
This biobrick is derived from M13mp18 phage vector, amplified by PCR. It includes 11 ORFs, M13 origin, a packaging sequence and Lac promoter. The promoter on the upstream of g2(gene 2) is deleted.The capsid is formed only when g2p(gene 2 protein) is expressed.
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You can insert the target gene or circuit on the downstream of the Lac promoter.
 
  
 
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Revision as of 21:02, 16 September 2013

Promoterless-M13 on pSB3

M13 is a filamentous phage that infects only F+ strains of E. coli, which does not kill the host cell. This biobrick is derived from M13mp18 phage vector, amplified by PCR. It includes 11 ORFs, M13 origin, a packaging sequence and Lac promoter. The promoter on the upstream of g2(gene 2) is deleted.The capsid is formed only when g2p(gene 2 protein) is expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 99
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6027
  • 1000
    COMPATIBLE WITH RFC[1000]