Difference between revisions of "Part:BBa I13605"

Latest revision as of 23:42, 5 July 2006

Reporter Construct (Ly-Tc-) for constitutive CFP and inducible YFP

Reporter plasmid with Lac operator+YFP(LVA-) reporter followed by a Tet operator+CFP(LVA-) reporter. Inducible with [http://openwetware.org/wiki/IPTG IPTG].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Pictures

[http://openwetware.org/wiki/IPTG IPTG] negative conditions	[http://openwetware.org/wiki/IPTG IPTG] positive condition
BBa_I13605 visualized under non-UV light BBa_I13605 visualized under 254nm UV lightbox	BBa_I13605 grown in IPTG visualized under 254nm UV lightbox

@@ Line 21: / Line 21: @@
 ! style = "background:#CCCCCC;"| <small>[http://openwetware.org/wiki/IPTG IPTG] negative conditions </small>
 ! style = "background:#CCCCCC;"| <small>[http://openwetware.org/wiki/IPTG IPTG] positive condition </small>
-! style = "background:#CCCCCC;"| <small>[http://openwetware.org/wiki/IPTG IPTG] positive (left) and negative (right)</small>
 |-
 |
@@ Line 28: / Line 27: @@
 ||
 [[Image:I13605 - UV 254nm plus IPTG.jpg|thumb|left|BBa_I13605 grown in IPTG visualized under 254nm UV lightbox]]
-|
-[[Image:I13605 - UV 254nm plus and minus IPTG.jpg|thumb|left|+(<font color = "red">left</font>) and -(<font color = "red">right</font>) [http://openwetware.org/wiki/IPTG IPTG]) visualized under 254nm wavelength UV lightbox]]
 |}