Difference between revisions of "Part:BBa K1164007"

Revision as of 07:07, 16 September 2013

pTRE driving yEGFP with tPGK1 terminator

This device consists of a modified version of the native Gal1 promoter found in S.cerevisiae driving production of yeast codon optimized GFP. The four gal4 binding domains present in the native Gal1 promoter have been replaced by four tetO sites. This promoter may be activated by regulatory activators that are able to bind to tetO. Upon activation, yEGFP will be produced.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 81
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1110

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1164007 short</partinfo>
-This device consists of a modified version of the native Gal1 promoter found in S.cerevisiae driving production of yeast codon optimized GFP. The four gal4 binding domains present in the native Gal1 promoter have been replaced by 4 tetO sites. This promoter may be activated by regulatory activators that are able to bind to the tetO sites. Upon activation, yEGFP will be produced.
+This device consists of a modified version of the native Gal1 promoter found in S.cerevisiae driving production of yeast codon optimized GFP. The four gal4 binding domains present in the native Gal1 promoter have been replaced by four tetO sites. This promoter may be activated by regulatory activators that are able to bind to tetO. Upon activation, yEGFP will be produced.